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CBX4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CBX4 Antibody (N-term) AP17028A
    Anti-CBX4 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CBX4 Antibody (N-term) AP17028A
    CBX4 Antibody (N-term) (Cat. #AP17028a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CBX4 antibody detected the CBX4 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00257
Other Accession O55187, NP_003646.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61368 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 8535
Other Names E3 SUMO-protein ligase CBX4, 632-, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4
Target/Specificity This CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBX4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBX4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX4
Function E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Cellular Location Nucleus. Nucleus speckle. Note=Localization to nuclear polycomb bodies is required for ZNF131 sumoylation
Tissue Location Ubiquitous.
Citations (0)

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E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.


Seong, I.S., et al. Hum. Mol. Genet. 19(4):573-583(2010)
Zhou, W., et al. BMB Rep 42(3):154-159(2009)
Liang, G., et al. Hum. Mol. Genet. 17(8):1109-1119(2008)
Kim, S.H., et al. Int. J. Biochem. Cell Biol. 40(11):2462-2471(2008)
Kabil, O., et al. Biochemistry 45(45):13528-13536(2006)

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$ 295.00
$ 99.00
Cat# AP17028A
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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