|Application ||WB, E|
|Other Accession||O55187, NP_003646.2|
|Calculated MW||61368 Da|
|Antigen Region||1-30 aa|
|Other Names||E3 SUMO-protein ligase CBX4, 632-, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4|
|Target/Specificity||This CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBX4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBX4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.|
|Cellular Location||Nucleus. Nucleus speckle. Note=Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Seong, I.S., et al. Hum. Mol. Genet. 19(4):573-583(2010)
Zhou, W., et al. BMB Rep 42(3):154-159(2009)
Liang, G., et al. Hum. Mol. Genet. 17(8):1109-1119(2008)
Kim, S.H., et al. Int. J. Biochem. Cell Biol. 40(11):2462-2471(2008)
Kabil, O., et al. Biochemistry 45(45):13528-13536(2006)
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