|Application ||WB, E|
|Other Accession||O55187, NP_003646.2|
|Calculated MW||61368 Da|
|Antigen Region||1-30 aa|
|Other Names||E3 SUMO-protein ligase CBX4, 632-, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4|
|Target/Specificity||This CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBX4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBX4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.|
|Cellular Location||Nucleus. Nucleus speckle. Note=Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880)|
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Provided below are standard protocols that you may find useful for product applications.
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Seong, I.S., et al. Hum. Mol. Genet. 19(4):573-583(2010)
Zhou, W., et al. BMB Rep 42(3):154-159(2009)
Liang, G., et al. Hum. Mol. Genet. 17(8):1109-1119(2008)
Kim, S.H., et al. Int. J. Biochem. Cell Biol. 40(11):2462-2471(2008)
Kabil, O., et al. Biochemistry 45(45):13528-13536(2006)
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