|Application ||WB, E|
|Other Accession||Q6PEE3, Q4R741, NP_001165948.1, NP_001165949.1|
|Calculated MW||40737 Da|
|Antigen Region||128-157 aa|
|Other Names||Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2, RRM2B, P53R2|
|Target/Specificity||This RRM2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human RRM2B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RRM2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage|
|Tissue Location||Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.
Zhou, B., et al. Mol. Cancer Ther. 9(6):1669-1679(2010)
Smith, P., et al. Biochemistry 48(46):11134-11141(2009)
Shaibani, A., et al. Arch. Neurol. 66(8):1028-1032(2009)
Tyynismaa, H., et al. Am. J. Hum. Genet. 85(2):290-295(2009)
Kollberg, G., et al. Neuromuscul. Disord. 19(2):147-150(2009)
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