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RNF10 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8N5U6 |
| Other Accession | NP_055683.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 89927 Da |
| Antigen Region | 178-206 aa |
| Gene ID | 9921 |
|---|---|
| Other Names | RING finger protein 10, RNF10, KIAA0262, RIE2 |
| Target/Specificity | This RNF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-206 amino acids from the N-terminal region of human RNF10. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNF10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF10 {ECO:0000303|PubMed:10697961, ECO:0000312|HGNC:HGNC:10055} |
|---|---|
| Function | E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling (PubMed:34348161, PubMed:34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome (PubMed:34348161, PubMed:34469731). Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation (PubMed:34348161). The action of RNF10 in iRQC is counteracted by USP10 (PubMed:34469731). May also act as a transcriptional factor involved in the regulation of MAG (Myelin- associated glycoprotein) expression (By similarity). Acts as a regulator of Schwann cell differentiation and myelination (By similarity). |
| Cellular Location | Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q5XI59} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known.
References
Hoshikawa, S., et al. PLoS ONE 3 (10), E3464 (2008) :
Stelzl, U., et al. Cell 122(6):957-968(2005)
Lin, J., et al. Mol. Cell. Biochem. 275 (1-2), 75-84 (2005) :
Seki, N., et al. J. Hum. Genet. 45(1):38-42(2000)
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