|Application ||WB, E|
|Calculated MW||67733 Da|
|Antigen Region||529-555 aa|
|Other Names||Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, MR-GEF, Related to Epac, Repac, RAPGEF5, GFR, KIAA0277, MRGEF|
|Target/Specificity||This RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-555 amino acids from the C-terminal region of human RAPGEF5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAPGEF5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GFR, KIAA0277, MRGEF|
|Function||Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.|
|Tissue Location||Widely expressed with highest levels in brain.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007) :
Rebhun, J.F., et al. J. Biol. Chem. 275(45):34901-34908(2000)
de Rooij, J., et al. J. Biol. Chem. 275(27):20829-20836(2000)
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