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P4HA1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P13674 |
| Other Accession | Q1RMU3, NP_001136067.1, NP_000908.2, NP_001017962.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 61049 Da |
| Antigen Region | 414-442 aa |
| Gene ID | 5033 |
|---|---|
| Other Names | Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA |
| Target/Specificity | This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | P4HA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | P4HA1 |
|---|---|
| Synonyms | P4HA |
| Function | Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
| Cellular Location | Endoplasmic reticulum lumen. |
| Tissue Location | Expressed in the heart, liver, skeletal muscle, kidney, placenta, lung and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Gorres, K.L., et al. PLoS ONE 4 (11), E7635 (2009) :
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006)
Grimmer, C., et al. Am. J. Pathol. 169(2):491-502(2006)
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Anonymous
2016-08-23 21:15:32
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Species tested
Human
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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