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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   P4HA1 Antibody (C-term)   

P4HA1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - P4HA1 Antibody (C-term) AP17086b
    P4HA1 Antibody (C-term) (Cat. #AP17086b) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the P4HA1 antibody detected the P4HA1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13674
Other Accession Q1RMU3, NP_001136067.1, NP_000908.2, NP_001017962.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61049 Da
Antigen Region 414-442 aa
Additional Information
Gene ID 5033
Other Names Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA
Target/Specificity This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsP4HA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name P4HA1
Synonyms P4HA
Function Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Cellular Location Endoplasmic reticulum lumen.
Research Areas
Citations (0)

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This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Gorres, K.L., et al. PLoS ONE 4 (11), E7635 (2009) :
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006)
Grimmer, C., et al. Am. J. Pathol. 169(2):491-502(2006)

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Species tested
Application tested
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose
$ 295.00
$ 99.00
Cat# AP17086b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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