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VDAC3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - VDAC3 Antibody (Center) AP17092c
    Overlay histogram showing Hela cells stained with AP17092c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17092c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - VDAC3 Antibody (Center) AP17092c
    All lanes : Anti-VDAC3 Antibody (Center) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: NCI-H460 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: Mouse testis lysate Lane 6: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - VDAC3 Antibody (Center) AP17092c
    All lanes : Anti-VDAC3 Antibody (Center) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: NCI-H460 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: Mouse testis lysate Lane 6: Mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - VDAC3 Antibody (Center) AP17092c
    VDAC3 Antibody (Center) (Cat. #AP17092c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the VDAC3 antibody detected the VDAC3 protein (arrow).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9Y277
Other Accession Q9TT13, Q29380, Q60931, Q9MZ13, NP_005653.3, NP_001129166.1
Reactivity Human, Mouse, Rat
Predicted Bovine, Pig, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30659 Da
Antigen Region 156-183 aa
Additional Information
Gene ID 7419
Other Names Voltage-dependent anion-selective channel protein 3, VDAC-3, hVDAC3, Outer mitochondrial membrane protein porin 3, VDAC3
Target/Specificity This VDAC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-183 amino acids from the Central region of human VDAC3.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVDAC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VDAC3
Function Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
Cellular Location Mitochondrion outer membrane.
Tissue Location Widely expressed. Highest in testis.
Research Areas
Citations ( 0 )

Background

VDAC3 belongs to a group of mitochondrial membrane channels involved in translocation of adenine nucleotides through the outer membrane. These channels may also function as a mitochondrial binding site for hexokinase (see HK1; MIM 142600) and glycerol kinase (GK; MIM 300474) (Rahmani et al., 1998).[supplied by OMIM].

References

Reina, S., et al. FEBS Lett. 584(13):2837-2844(2010)
Lefievre, L., et al. Proteomics 7(17):3066-3084(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

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$ 295.00
$ 99.00
Cat# AP17092c
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Quantity:
(40 western blots)
Availability: In Stock
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