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ARF5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ARF5 Antibody (Center) AP17093C
    ARF5 Antibody (Center) (Cat. #AP17093c) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARF5 antibody detected the ARF5 protein (arrow).
  • WB - ARF5 Antibody (Center) AP17093C
    Western blot analysis of ARF5 (arrow) using rabbit polyclonal ARF5 Antibody (Center) (Cat. #AP17093c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARF5 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P84085
Other Accession P84083, P84084, NP_001653.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20530 Da
Antigen Region 80-108 aa
Additional Information
Gene ID 381
Other Names ADP-ribosylation factor 5, ARF5
Target/Specificity This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARF5
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Cellular Location Golgi apparatus. Cytoplasm, perinuclear region
Research Areas
Citations (0)

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ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].


Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000)

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$ 295.00
$ 99.00
Cat# AP17093C
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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