|Application ||WB, E|
|Other Accession||P84083, P84084, NP_001653.1|
|Calculated MW||20530 Da|
|Antigen Region||80-108 aa|
|Other Names||ADP-ribosylation factor 5, ARF5|
|Target/Specificity||This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.|
|Cellular Location||Golgi apparatus. Cytoplasm, perinuclear region|
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Provided below are standard protocols that you may find useful for product applications.
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].
Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000)
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