|Application ||WB, E|
|Calculated MW||59232 Da|
|Antigen Region||216-244 aa|
|Other Names||Eyes absent homolog 2, EYA2, EAB1|
|Target/Specificity||This EYA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the Central region of human EYA2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EYA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:12500905, PubMed:23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed:12500905).|
|Cellular Location||Cytoplasm. Nucleus. Note=Retained in the cytoplasm via interaction with GNAZ and GNAI2 (PubMed:10906137). Interaction with SIX1, SIX2, SIX4 or SIX5 is required for translocation to the nucleus (PubMed:10906137, PubMed:12500905)|
|Tissue Location||Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Guo, J.T., et al. Zhonghua Zhong Liu Za Zhi 31(7):528-531(2009)
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