|Application ||WB, E|
|Other Accession||Q8K4F7, Q8MIZ3, Q9DAR7, Q8MJJ7, NP_054745.1|
|Predicted||Bovine, Mouse, Pig, Rat|
|Calculated MW||38609 Da|
|Antigen Region||165-193 aa|
|Other Names||m7GpppX diphosphatase, DCS-1, Decapping scavenger enzyme, Hint-related 7meGMP-directed hydrolase, Histidine triad nucleotide-binding protein 5, Histidine triad protein member 5, HINT-5, Scavenger mRNA-decapping enzyme DcpS, DCPS, DCS1, HINT5|
|Target/Specificity||This DCPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human DCPS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DCPS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre- mRNAs. Inhibits activation-induced cell death.|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly localized in the nucleus. Nucleocytoplasmic shuttling protein that can transiently enter the cytoplasm in mammalian cells in a XPO1/CRM1-dependent manner|
|Tissue Location||Detected in liver, brain, kidney, testis and prostate.|
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Provided below are standard protocols that you may find useful for product applications.
Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides.
Sebastiani, P., et al. Science (2010) In press :
Mariller, C., et al. Biochimie 91(1):109-122(2009)
Liu, S.W., et al. J. Biol. Chem. 283(24):16427-16436(2008)
Shen, V., et al. RNA 14(6):1132-1142(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
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