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GCNT1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q02742 |
| Other Accession | NP_001091103.1, NP_001091102.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49799 Da |
| Antigen Region | 63-91 aa |
| Gene ID | 2650 |
|---|---|
| Other Names | Beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase, Core 2-branching enzyme, Core2-GlcNAc-transferase, C2GNT, Core 2 GNT, GCNT1, NACGT2 |
| Target/Specificity | This GCNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the Central region of human GCNT1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GCNT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GCNT1 |
|---|---|
| Synonyms | NACGT2 |
| Function | Glycosyltransferase that catalyzes the transfer of an N- acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan (PubMed:1329093, PubMed:23027862). The catalysis is metal ion- independent and occurs with inversion of the anomeric configuration of sugar donor (By similarity). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (By similarity). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage- specific embryonic antigen 1 (SSEA-1) determinant (By similarity). Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates (By similarity). |
| Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein. Note=Also detected in the trans-Golgi network |
| Tissue Location | Highly expressed in activated T-lymphocytes and myeloid cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified.
References
Hatakeyama, S., et al. Int. J. Cancer 127(5):1052-1059(2010)
Brockhausen, I., et al. Biochim. Biophys. Acta 1790(10):1244-1257(2009)
St Hill, C.A., et al. BMC Cancer 9, 79 (2009) :
Nagaraj, S., et al. Pancreas 37(3):321-327(2008)
Julien, S., et al. J. Immunol. 179(9):5701-5710(2007)
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