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ASF1A Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ASF1A Antibody (C-term) AP17195B
    ASF1A Antibody (C-term) (Cat. #AP17195b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ASF1A antibody detected the ASF1A protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y294
Other Accession Q3C1E9, Q9CQE6, Q2KIG1, NP_054753.1
Reactivity Human
Predicted Bovine, Mouse, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22969 Da
Antigen Region 140-168 aa
Additional Information
Gene ID 25842
Other Names Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, hAsf1, hAsf1a, CCG1-interacting factor A, CIA, hCIA, ASF1A
Target/Specificity This ASF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ASF1A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASF1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASF1A {ECO:0000303|PubMed:10759893, ECO:0000312|HGNC:HGNC:20995}
Function Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:10759893, PubMed:11897662, PubMed:12842904, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198). Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks: acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51 (PubMed:29478807). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:21454524, PubMed:29408485). Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit (PubMed:15621527).
Cellular Location Nucleus. Chromosome
Tissue Location Ubiquitously expressed.
Citations (0)
citation

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Background

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq].

References

Jasencakova, Z., et al. Mol. Cell 37(5):736-743(2010)
Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009)
Banumathy, G., et al. Mol. Cell. Biol. 29(3):758-770(2009)
Ambagala, A.P., et al. J. Virol. 83(1):200-209(2009)
Pilyugin, M., et al. PLoS ONE 4 (12), E8328 (2009) :

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$ 182.50
$ 70.00
Cat# AP17195B
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