|Application ||WB, E|
|Other Accession||Q4R945, NP_003309.2|
|Calculated MW||97072 Da|
|Antigen Region||6-35 aa|
|Other Names||Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT, TTK, MPS1, MPS1L1|
|Target/Specificity||This TTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-35 amino acids from the N-terminal region of human TTK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TTK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.|
|Tissue Location||Present in rapidly proliferating cell lines.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
Cui, Y., et al. J. Biol. Chem. 285(43):32988-32998(2010)
Maciejowski, J., et al. J. Cell Biol. 190(1):89-100(2010)
Santaguida, S., et al. J. Cell Biol. 190(1):73-87(2010)
Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010)
Lan, W., et al. J. Cell Biol. 190(1):21-24(2010)
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