|Application ||WB, E|
|Other Accession||Q3KR59, P52479, Q5ZJN4, A5PJS6, Q7ZXR7, Q2NL57, NP_005144.2|
|Predicted||Xenopus, Bovine, Chicken, Mouse, Rat|
|Calculated MW||87134 Da|
|Antigen Region||500-529 aa|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thioesterase 10, Ubiquitin-specific-processing protease 10, USP10, KIAA0190|
|Target/Specificity||This USP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-529 amino acids from the Central region of human USP10.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USP10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.|
|Cellular Location||Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. [provided by RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yuan, J., et al. Cell 140(3):384-396(2010)
Bomberger, J.M., et al. J. Biol. Chem. 284(28):18778-18789(2009)
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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