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SGOL1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP17206c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP17206c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
SGOL1 Antibody (Center) - Product info | |
| Application | WB
|
| Primary Accession | Q5FBB7 |
| Other Accession | NP_001012409.1, NP_001012410.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 64190 Da |
SGOL1 Antibody (Center) - Additional info | |
| Gene ID 151648 | |
| Other Names SGOL1; SGO1; Shugoshin-like 1; Serologically defined breast cancer antigen NY-BR-85 | |
| Target/Specificity This SGOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-367 amino acids from the Central region of human SGOL1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SGOL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SGOL1 Antibody (Center) - Protein Information | |
| Name SGOL1 | |
| Synonyms SGO1 | |
| Function Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity | |
| Cellular Location Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle | |
| Tissue Location Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta Strongly overexpressed in 90% of breast cancers tested | |
SGOL1 Antibody (Center) - Related products
SGOL1 Antibody (Center) - Application data
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SGOL1 Antibody (Center) (Cat. #AP17206c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SGOL1 antibody detected the SGOL1 protein (arrow).
SGOL1 Antibody (Center) - Research Areas
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BACKGROUND
SGOL1 plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
REFERENCES
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Okamoto, N., et al. Genes Cells 15(5):471-484(2010) Xu, Z., et al. Mol. Cell 35(4):426-441(2009) Gambe, A.E., et al. FEBS Lett. 583(12):1951-1956(2009) Karamysheva, Z., et al. J. Biol. Chem. 284(3):1772-1780(2009)
