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POLR3C Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLR3C Antibody (Center) AP17225c
    POLR3C Antibody (Center) (Cat. #AP17225c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR3C antibody detected the POLR3C protein (arrow).
  • WB - POLR3C Antibody (Center) AP17225c
    Western blot analysis of POLR3C (arrow) using rabbit polyclonal POLR3C Antibody (Center) (Cat. #AP17225c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR3C gene.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BUI4
Other Accession NP_006459.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60612 Da
Antigen Region 202-230 aa
Additional Information
Gene ID 10623
Other Names DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit C, RNA polymerase III 62 kDa subunit, RPC62, POLR3C
Target/Specificity This POLR3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of human POLR3C.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR3C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR3C
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Cellular Location Nucleus.
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

References

Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009)
Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002)
Kuwana, M., et al. Arthritis Rheum. 46(10):2742-2747(2002)
Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999)
Hsieh, Y.J., et al. Mol. Cell. Biol. 19(7):4944-4952(1999)

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$ 295.00
$ 99.00
Cat# AP17225c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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