|Application ||WB, E|
|Other Accession||NP_002501.1, NP_001005340.1|
|Calculated MW||63923 Da|
|Antigen Region||541-569 aa|
|Other Names||Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB|
|Target/Specificity||This GPNMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-569 amino acids from the C-terminal region of human GPNMB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GPNMB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Could be a melanogenic enzyme.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines|
|Tissue Location||Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Williams, M.D., et al. Melanoma Res. 20(3):184-190(2010)
Hoashi, T., et al. FASEB J. 24(5):1616-1629(2010)
Rose, A.A., et al. Clin. Cancer Res. 16(7):2147-2156(2010)
Rose, A.A., et al. PLoS ONE 5 (8), E12093 (2010) :
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