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ALG2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ALG2 Antibody (Center) AP17233c
    ALG2 Antibody (Center) (Cat. #AP17233c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ALG2 antibody detected the ALG2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H553
Other Accession NP_149078.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47092 Da
Antigen Region 254-282 aa
Additional Information
Gene ID 85365
Other Names Alpha-1, 3/1, 6-mannosyltransferase ALG2, Asparagine-linked glycosylation protein 2 homolog, GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1, 6-mannosyltransferase, ALG2
Target/Specificity This ALG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-282 amino acids from the Central region of human ALG2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALG2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALG2
Function Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.
Cellular Location Membrane; Single-pass membrane protein
Research Areas
Citations (0)
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Background

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants.

References

Inuzuka, T., et al. BMC Struct. Biol. 10, 25 (2010) :
Okumura, M., et al. Biochem. Biophys. Res. Commun. 386(1):237-241(2009)
Hoj, B.R., et al. Biochem. Biophys. Res. Commun. 378(1):145-148(2009)
Mahul-Mellier, A.L., et al. J. Biol. Chem. 283(50):34954-34965(2008)
la Cour, J.M., et al. Mol Oncol 1(4):431-439(2008)

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$ 182.50
$ 70.00
Cat# AP17233c
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