- CITATIONS: 2
|Application ||WB, IHC-P, FC, E|
|Calculated MW||83134 Da|
|Antigen Region||521-554 aa|
|Other Names||Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, HGF, HPTA|
|Target/Specificity||This HGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from the C-terminal region of human HGF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HGF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.|
Provided below are standard protocols that you may find useful for product applications.
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.
Ryugo, M., et al., Transplantation 78(8):1153-1158 (2004).
Lyon, M., et al., J. Biol. Chem. 279(42):43560-43567 (2004).
He, Y., et al., World J. Gastroenterol. 10(19):2827-2830 (2004).
Tjin, E.P., et al., Blood 104(7):2172-2175 (2004).
Matsuda-Hashii, Y., et al., Exp. Hematol. 32(10):955-961 (2004).
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