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COL8A1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - COL8A1 Antibody (N-term) AP17250a
    All lanes : Anti-COL8A1 Antibody (N-term) at 1:1000 dilution Lane 1: human heart lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - COL8A1 Antibody (N-term) AP17250a
    COL8A1 Antibody (N-term) (Cat. #AP17250a) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the COL8A1 antibody detected the COL8A1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27658
Other Accession NP_001841.2, NP_065084.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73364 Da
Antigen Region 62-89 aa
Additional Information
Gene ID 1295
Other Names Collagen alpha-1(VIII) chain, Endothelial collagen, Vastatin, COL8A1, C3orf7
Target/Specificity This COL8A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the N-terminal region of human COL8A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL8A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL8A1
Synonyms C3orf7
Function Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
Research Areas
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This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same isoform have been observed.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Neale, B.M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7395-7400(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)

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$ 295.00
$ 99.00
Cat# AP17250a
(40 western blots)
Availability: In Stock
Bulk Size
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