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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   TCF25 Antibody (N-term)   

TCF25 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - TCF25 Antibody (N-term) AP17279A
    Fluorescent image of A549 cell stained with TCF25 Antibody (N-term)(Cat#AP17279a).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).TCF25 immunoreactivity is localized to Cytoplasm significantly.
  • WB - TCF25 Antibody (N-term) AP17279A
    TCF25 Antibody (N-term) (Cat. #AP17279a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BQ70
Other Accession Q8R3L2, NP_055787.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 76667 Da
Antigen Region 186-215 aa
Additional Information
Gene ID 22980
Other Names Transcription factor 25, TCF-25, Nuclear localized protein 1, TCF25, KIAA1049, NULP1
Target/Specificity This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCF25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF25
Synonyms KIAA1049, NULP1
Function May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
Cellular Location Nucleus. Note=Some staining in the cytosol
Tissue Location In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
Research Areas
Citations (0)

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TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM].


Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Steen, H., et al. Biochem. Biophys. Res. Commun. 366(2):432-437(2008)
Cai, Z., et al. Biochem. Biophys. Res. Commun. 343(3):973-981(2006)

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$ 295.00
$ 99.00
Cat# AP17279A
(40 western blots)
Availability: In Stock
Bulk Size
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