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PLEKHG5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O94827 |
| Other Accession | Q6RFZ7, Q66T02, NP_001036128.1, NP_001036129.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 111231 Da |
| Antigen Region | 175-202 aa |
| Gene ID | 57449 |
|---|---|
| Other Names | Pleckstrin homology domain-containing family G member 5, PH domain-containing family G member 5, Guanine nucleotide exchange factor 720, GEF720, PLEKHG5, KIAA0720 |
| Target/Specificity | This PLEKHG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-202 amino acids from the N-terminal region of human PLEKHG5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PLEKHG5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PLEKHG5 |
|---|---|
| Synonyms | KIAA0720 |
| Function | Functions as a guanine exchange factor (GEF) for RAB26 and thus regulates autophagy of synaptic vesicles in axon terminal of motoneurons (By similarity). Involved in the control of neuronal cell differentiation (PubMed:11704860). Plays a role in angiogenesis through regulation of endothelial cells chemotaxis. Affects also the migration, adhesion, and matrix/bone degradation in macrophages and osteoclasts (PubMed:23777631). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q66T02}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q66T02}. Cell membrane {ECO:0000250|UniProtKB:Q66T02}. Cell junction {ECO:0000250|UniProtKB:Q66T02}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q66T02}. Note=Predominantly cytoplasmic, however when endothelial cells are stimulated with lysophosphatidic acid, PLEKHG5 is found in perinuclear regions and at the cell membrane Localizes at cell-cell junctions in quiescent endothelial cells, and relocalizes to cytoplasmic vesicle and the leading edge of lamellipodia in migrating endothelial cells. {ECO:0000250|UniProtKB:Q66T02} |
| Tissue Location | Predominantly expressed in the peripheral nervous system and brain. Highest expression is observed in heart, lung, kidney, testis and moderate expression is present in spleen, pancreas, skeletal muscle, ovary and liver. Weakly expressed in glioblastoma (GBM) cell lines. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein that activates the nuclear factor kappa B (NFKB1) signaling pathway. Multations in this gene have been found in a family with distal spinal muscular atrophy.
References
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009)
Maystadt, I., et al. Am. J. Hum. Genet. 81(1):67-76(2007)
Rabizadeh, S., et al. Cytokine Growth Factor Rev. 14 (3-4), 225-239 (2003) :
Matsuda, A., et al. Oncogene 22(21):3307-3318(2003)
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