NARF Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9UHQ1 |
Other Accession | NP_001033707.1, NP_001077077.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 51156 Da |
Antigen Region | 134-160 aa |
Gene ID | 26502 |
---|---|
Other Names | Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2, IOP2, NARF |
Target/Specificity | This NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human NARF. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NARF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NARF |
---|---|
Cellular Location | Nucleus. |
Tissue Location | Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Moller-Krull, M., et al. J. Mol. Biol. 382(3):601-609(2008)
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Lattanzi, G., et al. J. Cell. Biochem. 102(5):1149-1159(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
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