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MSL2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MSL2 Antibody (N-term) AP17353a
    Anti-MSL2 Antibody (N-term) at 1:1000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9HCI7
Other Accession Q69ZF8, NP_001138889.1, NP_060603.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62541 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 55167
Other Names E3 ubiquitin-protein ligase MSL2, 632-, Male-specific lethal 2-like 1, MSL2-like 1, Male-specific lethal-2 homolog, MSL-2, Male-specific lethal-2 homolog 1, RING finger protein 184, MSL2, KIAA1585, MSL2L1, RNF184
Target/Specificity This MSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MSL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSL2
Synonyms KIAA1585, MSL2L1, RNF184
Function Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histone H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.
Citations (0)
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Background

Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure.

References

Dehghan, A., et al. Circ Cardiovasc Genet 2(2):125-133(2009)
Mendjan, S., et al. Mol. Cell 21(6):811-823(2006)
Smith, E.R., et al. Mol. Cell. Biol. 25(21):9175-9188(2005)
Marin, I. J. Mol. Evol. 56(5):527-539(2003)
Lyman, L.M., et al. Genetics 147(4):1743-1753(1997)

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$ 182.50
$ 70.00
Cat# AP17353a
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