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UBE2T Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - UBE2T Antibody (C-term) AP17379b
    Western blot analysis of UBE2T (arrow) using rabbit polyclonal UBE2T Antibody (C-term) (Cat. #AP17379b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NPD8
Other Accession Q32LD2, NP_054895.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22521 Da
Antigen Region 126-154 aa
Additional Information
Gene ID 29089
Other Names Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T
Target/Specificity This UBE2T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the C-terminal region of human UBE2T.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2T Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2T
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
Cellular Location Nucleus. Note=Accumulates to chromatin
Research Areas
Citations (0)

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The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).


Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)
Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)
Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)
Hao, J., et al. Tumour Biol. 29(3):195-203(2008)
Machida, Y.J., et al. Mol. Cell 23(4):589-596(2006)

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$ 295.00
$ 99.00
Cat# AP17379b
(40 western blots)
Availability: In Stock
Bulk Size
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