|Application ||WB, E|
|Other Accession||Q32LD2, NP_054895.1|
|Calculated MW||22521 Da|
|Antigen Region||126-154 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T|
|Target/Specificity||This UBE2T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the C-terminal region of human UBE2T.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2T Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.|
|Cellular Location||Nucleus. Note=Accumulates to chromatin|
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Provided below are standard protocols that you may find useful for product applications.
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).
Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)
Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)
Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)
Hao, J., et al. Tumour Biol. 29(3):195-203(2008)
Machida, Y.J., et al. Mol. Cell 23(4):589-596(2006)
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