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>   home   >   Products   >   Primary Antibodies   >   AKAP8L Antibody (C-term)   

AKAP8L Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AKAP8L Antibody (C-term) AP17416B
    AKAP8L Antibody (C-term) (Cat. #AP17416b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).
  • WB - AKAP8L Antibody (C-term) AP17416B
    AKAP8L Antibody (C-term) (Cat. #AP17416b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9ULX6
Other Accession Q9R0L7, NP_055186.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71649 Da
Antigen Region 485-512 aa
Additional Information
Gene ID 26993
Other Names A-kinase anchor protein 8-like, AKAP8-like protein, Helicase A-binding protein 95, HAP95, Homologous to AKAP95 protein, HA95, Neighbor of A-kinase-anchoring protein 95, Neighbor of AKAP95, AKAP8L, NAKAP, NAKAP95
Target/Specificity This AKAP8L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 485-512 amino acids from the C-terminal region of human AKAP8L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKAP8L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKAP8L
Synonyms NAKAP, NAKAP95
Function Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).
Cellular Location Nucleus. Nucleus matrix Nucleus speckle. Nucleus, PML body. Cytoplasm. Note=Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899) Colocalizes with EBNA-LP in PML bodies (PubMed:11884601)
Tissue Location Ubiquitously expressed. Expressed in the brain cortex (at protein level).
Citations (0)

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Background

AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.

References

Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008)
Kvissel, A.K., et al. Exp. Cell Res. 313(13):2795-2809(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Sayer, J.A., et al. Neuromolecular Med. 7(4):297-310(2005)

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$ 295.00
$ 99.00
Cat# AP17416B
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Quantity:
(40 western blots)
Availability: In Stock
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