|Application ||WB, E|
|Other Accession||Q9R0L7, NP_055186.2|
|Calculated MW||71649 Da|
|Antigen Region||485-512 aa|
|Other Names||A-kinase anchor protein 8-like, AKAP8-like protein, Helicase A-binding protein 95, HAP95, Homologous to AKAP95 protein, HA95, Neighbor of A-kinase-anchoring protein 95, Neighbor of AKAP95, AKAP8L, NAKAP, NAKAP95|
|Target/Specificity||This AKAP8L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 485-512 amino acids from the C-terminal region of human AKAP8L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AKAP8L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).|
|Cellular Location||Nucleus. Nucleus matrix Nucleus speckle. Nucleus, PML body. Cytoplasm. Note=Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899) Colocalizes with EBNA-LP in PML bodies (PubMed:11884601)|
|Tissue Location||Ubiquitously expressed. Expressed in the brain cortex (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008)
Kvissel, A.K., et al. Exp. Cell Res. 313(13):2795-2809(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Sayer, J.A., et al. Neuromolecular Med. 7(4):297-310(2005)
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