|Application ||WB, E|
|Other Accession||O89044, NP_000938.2|
|Calculated MW||58806 Da|
|Antigen Region||317-345 aa|
|Other Names||DNA primase large subunit, 277-, DNA primase 58 kDa subunit, p58, PRIM2, PRIM2A|
|Target/Specificity||This PRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-345 amino acids from the Central region of human PRIM2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRIM2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.|
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Provided below are standard protocols that you may find useful for product applications.
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit. [provided by RefSeq].
Vaithiyalingam, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13684-13689(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Weiner, B.E., et al. J. Biol. Chem. 282(46):33444-33451(2007)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
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