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ARID5B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q14865 |
| Other Accession | Q8BM75, Q5ZJ69, E1BLP6, NP_115575.1 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 132375 Da |
| Antigen Region | 389-418 aa |
| Gene ID | 84159 |
|---|---|
| Other Names | AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2, ARID5B, DESRT, MRF2 |
| Target/Specificity | This ARID5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the Central region of human ARID5B. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARID5B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARID5B |
|---|---|
| Synonyms | DESRT, MRF2 |
| Function | Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00355}. |
| Tissue Location | Widely expressed, including in liver (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
Background
DNA-binding protein that binds to the 5'-AATA[CT]-3' core sequence. Probably acts as a transcription regulator. Represses the cytomegalovirus enhancer. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may act as a regulator of smooth muscle cell differentiation and proliferation. May be involved in lipid stores.
References
Healy, J., et al. Haematologica 95(9):1608-1611(2010)
Yang, W., et al. Leukemia 24(4):894-896(2010)
Prasad, R.B., et al. Blood 115(9):1765-1767(2010)
Papaemmanuil, E., et al. Nat. Genet. 41(9):1006-1010(2009)
Trevino, L.R., et al. Nat. Genet. 41(9):1001-1005(2009)
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