HECTD3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q5T447 |
Other Accession | NP_078878.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 97113 Da |
Antigen Region | 740-769 aa |
Gene ID | 79654 |
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Other Names | E3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3 |
Target/Specificity | This HECTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 740-769 amino acids from the C-terminal region of human HECTD3. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HECTD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HECTD3 |
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Function | E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity). |
Cellular Location | Cytoplasm, perinuclear region. |
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
References
Yu, J., et al. Biochem. Biophys. Res. Commun. 367(4):805-812(2008)
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
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