|Application ||WB, E|
|Calculated MW||26153 Da|
|Antigen Region||87-114 aa|
|Other Names||Kinetochore protein Spc25, hSpc25, SPC25, SPBC25|
|Target/Specificity||This SPC25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the Central region of human SPC25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPC25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
Pare, G., et al. PLoS Genet. 4 (12), E1000312 (2008) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ciferri, C., et al. J. Biol. Chem. 280(32):29088-29095(2005)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Bharadwaj, R., et al. J. Biol. Chem. 279(13):13076-13085(2004)
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