|Application ||WB, E|
|Calculated MW||26153 Da|
|Antigen Region||87-114 aa|
|Other Names||Kinetochore protein Spc25, hSpc25, SPC25, SPBC25|
|Target/Specificity||This SPC25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the Central region of human SPC25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPC25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
Pare, G., et al. PLoS Genet. 4 (12), E1000312 (2008) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ciferri, C., et al. J. Biol. Chem. 280(32):29088-29095(2005)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Bharadwaj, R., et al. J. Biol. Chem. 279(13):13076-13085(2004)
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