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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SPC25 Antibody (Center)   

SPC25 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPC25 Antibody (Center) AP17479C
    SPC25 Antibody (Center) (Cat. #AP17479c) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SPC25 antibody detected the SPC25 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HBM1
Other Accession NP_065726.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 26153 Da
Antigen Region 87-114 aa
Additional Information
Gene ID 57405
Other Names Kinetochore protein Spc25, hSpc25, SPC25, SPBC25
Target/Specificity This SPC25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the Central region of human SPC25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPC25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPC25
Synonyms SPBC25
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore
Research Areas
Citations (0)

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This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.


Pare, G., et al. PLoS Genet. 4 (12), E1000312 (2008) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ciferri, C., et al. J. Biol. Chem. 280(32):29088-29095(2005)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Bharadwaj, R., et al. J. Biol. Chem. 279(13):13076-13085(2004)

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Cat# AP17479C
(40 western blots)
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