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CHST10 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O43529 |
| Other Accession | NP_004845.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42207 Da |
| Antigen Region | 208-236 aa |
| Gene ID | 9486 |
|---|---|
| Other Names | Carbohydrate sulfotransferase 10, 282-, HNK-1 sulfotransferase, HNK-1ST, HNK1ST, HuHNK-1ST, CHST10 |
| Target/Specificity | This CHST10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the C-terminal region of human CHST10. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CHST10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHST10 {ECO:0000303|PubMed:23269668, ECO:0000312|HGNC:HGNC:19650} |
|---|---|
| Function | Catalyzes the transfer of sulfate from 3'-phosphoadenylyl sulfate (PAPS) to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure 3-O-sulfo-beta-D-GlcA- (1->3)-beta-D-Gal-(1->4)-D-GlcNAc-R, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis (PubMed:9478973). Sulfates terminal glucuronyl residue of the laminin globular (LG)-domain binding epitope on DAG1/alpha-dystroglycan and prevents further polymerization by LARGE1 glycosyltransferase. Likely defines the chain length of LG epitope, conferring binding specificity to extracellular matrix components (PubMed:32149355). Plays a role in down-regulating the steroid hormones. Sulfates glucuronidated estrogens and androgens with an impact in hormone cycle and fertility. Has a preference for glucuronyl moiety at the 3-hydroxyl group of a sterol ring rather than the 17-hydroxyl group, showing high catalytic efficiency for 17beta-estradiol 3-O-(beta-D-glucuronate) and dehydroepiandrosterone 3-O-(beta-D-glucuronate) hormones (PubMed:23269668). |
| Cellular Location | Golgi apparatus membrane {ECO:0000250|UniProtKB:O54702}; Single-pass type II membrane protein |
| Tissue Location | In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cell surface carbohydrates modulate a variety of cellular functions and are typically synthesized in a stepwise manner. HNK1ST plays a role in the biosynthesis of HNK1 (see MIM 151290), a neuronally expressed carbohydrate that contains a sulfoglucuronyl residue.
References
Zhao, X., et al. Cancer Res. 69(12):5218-5225(2009)
Kang, H.G., et al. J. Biol. Chem. 277(38):34766-34772(2002)
Ong, E., et al. J. Biol. Chem. 274(36):25608-25612(1999)
Ong, E., et al. J. Biol. Chem. 273(9):5190-5195(1998)
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