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ARFGAP2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARFGAP2 Antibody (Center) AP17535c
    ARFGAP2 Antibody (Center) (Cat. #AP17535c) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARFGAP2 antibody detected the ARFGAP2 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8N6H7
Other Accession Q3MID3, Q99K28, A1L520, NP_115765.2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56720 Da
Antigen Region 276-305 aa
Additional Information
Gene ID 84364
Other Names ADP-ribosylation factor GTPase-activating protein 2, ARF GAP 2, GTPase-activating protein ZNF289, Zinc finger protein 289, ARFGAP2, ZNF289
Target/Specificity This ARFGAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the Central region of human ARFGAP2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARFGAP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARFGAP2
Synonyms ZNF289
Function GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment
Citations (0)

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Background

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

References

Saitoh, A., et al. J. Biol. Chem. 284(20):13948-13957(2009)
Weimer, C., et al. J. Cell Biol. 183(4):725-735(2008)
Frigerio, G., et al. Traffic 8(11):1644-1655(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Schmid, E.M., et al. PLoS Biol. 4 (9), E262 (2006) :

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$ 295.00
$ 99.00
Cat# AP17535c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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