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IMPACT Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IMPACT Antibody (N-term) AP17553a
    IMPACT Antibody (N-term) (Cat. #AP17553a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9P2X3
Other Accession Q5GFD8, NP_060909.1
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36476 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 55364
Other Names Protein IMPACT, Imprinted and ancient gene protein homolog, IMPACT
Target/Specificity This IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIMPACT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IMPACT
Function Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1- mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O55091}.
Tissue Location Widely expressed. Expressed at high level in brain.
Citations (0)

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Background

Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity).

References

Habibi, D., et al. J. Cell. Physiol. 225(1):196-205(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Pereira, C.M., et al. J. Biol. Chem. 280(31):28316-28323(2005)
Okamura, K., et al. Biochem. Biophys. Res. Commun. 329(3):824-830(2005)
Okamura, K., et al. DNA Res. 11(6):381-390(2004)

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$ 295.00
$ 99.00
Cat# AP17553a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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