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IMPACT Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9P2X3 |
| Other Accession | Q5GFD8, NP_060909.1 |
| Reactivity | Human |
| Predicted | Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 36476 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 55364 |
|---|---|
| Other Names | Protein IMPACT, Imprinted and ancient gene protein homolog, IMPACT |
| Target/Specificity | This IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IMPACT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IMPACT |
|---|---|
| Function | Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:O55091}. |
| Tissue Location | Widely expressed. Expressed at high level in brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity).
References
Habibi, D., et al. J. Cell. Physiol. 225(1):196-205(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Pereira, C.M., et al. J. Biol. Chem. 280(31):28316-28323(2005)
Okamura, K., et al. Biochem. Biophys. Res. Commun. 329(3):824-830(2005)
Okamura, K., et al. DNA Res. 11(6):381-390(2004)
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