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CRB3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BUF7 |
| Other Accession | Q8QZT4, NP_631900.1, NP_777377.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 12854 Da |
| Antigen Region | 67-95 aa |
| Gene ID | 92359 |
|---|---|
| Other Names | Protein crumbs homolog 3, CRB3 |
| Target/Specificity | This CRB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the Central region of human CRB3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CRB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CRB3 (HGNC:20237) |
|---|---|
| Function | Involved in the establishment of cell polarity in mammalian epithelial cells (PubMed:12771187, PubMed:14718572). Regulates the morphogenesis of tight junctions (PubMed:12771187, PubMed:14718572). Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity). |
| Cellular Location | Apical cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells. |
| Tissue Location | Preferentially expressed in epithelial tissues (PubMed:14718572). Expressed at high levels in lung, kidney, and colon (PubMed:12527193, PubMed:14718572). Expressed at high levels in retina, colon and mammary glands (PubMed:12527193). Moderately expressed in liver, spleen, pancreas and prostate (PubMed:12527193). Moderately to weakly expressed in the placenta (PubMed:14718572, PubMed:12527193) Weakly expressed in skeletal muscle and small intestine (PubMed:14718572). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
References
Pardossi-Piquard, R., et al. Biochemistry 46(48):13704-13710(2007)
Fan, S., et al. J. Cell Biol. 178(3):387-398(2007)
Fogg, V.C., et al. J. Cell. Sci. 118 (PT 13), 2859-2869 (2005) :
Lemmers, C., et al. Mol. Biol. Cell 15(3):1324-1333(2004)
Roh, M.H., et al. J. Cell. Sci. 116 (PT 14), 2895-2906 (2003) :
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