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PNPT1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PNPT1 Antibody (Center) AP17563c
    PNPT1 Antibody (Center) (Cat. #AP17563c) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).
  • WB - PNPT1 Antibody (Center) AP17563c
    PNPT1 Antibody (Center) (Cat. #AP17563c) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TCS8
Other Accession Q8K1R3, NP_149100.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85951 Da
Antigen Region 284-311 aa
Additional Information
Gene ID 87178
Other Names Polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein, PNPT1, PNPASE
Target/Specificity This PNPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-311 amino acids from the Central region of human PNPT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPNPT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PNPT1
Synonyms PNPASE
Function RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double- stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.
Cellular Location Cytoplasm. Mitochondrion. Mitochondrion intermembrane space; Peripheral membrane protein
Research Areas
Citations (0)

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PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).


Wang, G., et al. Cell 142(3):456-467(2010)
Das, S.K., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11948-11953(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
O'Doherty, C., et al. Pharmacogenomics 10(7):1177-1186(2009)
Luczynski, W., et al. Neoplasma 56(5):428-434(2009)

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Cat# AP17563c
(40 western blots)
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