|Application ||WB, E|
|Other Accession||Q4R7H4, NP_057037.1|
|Calculated MW||33172 Da|
|Antigen Region||67-95 aa|
|Other Names||Nitric oxide synthase-interacting protein, eNOS-interacting protein, NOSIP|
|Target/Specificity||This NOSIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human NOSIP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NOSIP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).|
|Cellular Location||Cytoplasm. Nucleus Note=Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813)|
|Tissue Location||Expressed in heart, brain and lung. Present in endothelial cells (at protein level)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
NOSIP negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Schleicher, M., et al. Mol. Cell. Biol. 25(18):8251-8258(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Dreyer, J., et al. J. Neurosci. 24(46):10454-10465(2004)
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