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RNF138 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNF138 Antibody (Center) AP17600C
    RNF138 Antibody (Center) (Cat. #AP17600c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the RNF138 antibody detected the RNF138 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8WVD3
Other Accession Q32LN5, NP_057355.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28193 Da
Antigen Region 76-103 aa
Additional Information
Gene ID 51444
Other Names E3 ubiquitin-protein ligase RNF138, 632-, Nemo-like kinase-associated RING finger protein, NLK-associated RING finger protein, hNARF, RING finger protein 138, RNF138, NARF
Target/Specificity This RNF138 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the Central region of human RNF138.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF138 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF138 (HGNC:17765)
Function E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination (PubMed:26502055, PubMed:26502057). Recruited to sites of double- strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination (PubMed:26502055, PubMed:26502057). Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination (PubMed:26502055). According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP (PubMed:26502057). Together with NLK, involved in the ubiquitination and degradation of TCF/LEF (PubMed:16714285). Also exhibits auto-ubiquitination activity in combination with UBE2K (PubMed:16714285). May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (PubMed:16714285).
Cellular Location Chromosome. Note=Recruited at DNA damage sites (PubMed:26502055). Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (PubMed:26502055, PubMed:26502057)
Research Areas
Citations (0)

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Background

The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Yamada, M., et al. J. Biol. Chem. 281(30):20749-20760(2006)
Lim, J., et al. Cell 125(4):801-814(2006)
Saurin, A.J., et al. Trends Biochem. Sci. 21(6):208-214(1996)

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$ 295.00
$ 99.00
Cat# AP17600C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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