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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PRRT2 Antibody (Center)   

PRRT2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - PRRT2 Antibody (Center) AP17642c
    Overlay histogram showing U-2 OS cells stained with AP17642c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17642c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - PRRT2 Antibody (Center) AP17642c
    Anti-PRRT2 Antibody (Center) at 1:1000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PRRT2 Antibody (Center) AP17642c
    Anti-PRRT2 Antibody (Center) at 1:2000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PRRT2 Antibody (Center) AP17642c
    PRRT2 Antibody (Center) (Cat. #AP17642c) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PRRT2 antibody detected the PRRT2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q7Z6L0
Other Accession NP_660282.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34945 Da
Antigen Region 207-233 aa
Additional Information
Gene ID 112476
Other Names Proline-rich transmembrane protein 2, Dispanin subfamily B member 3, DSPB3, PRRT2
Target/Specificity This PRRT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-233 amino acids from the Central region of human PRRT2.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRRT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRRT2
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, synapse
Research Areas
Citations (0)

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Background

PRRT2 belongs to the CD225 family. There are 3 named isoforms of PRRT2 produced by alternative splicing.

References

Lamesch, P., et al. Genomics 89(3):307-315(2007)

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$ 295.00
$ 99.00
Cat# AP17642c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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