|Application ||WB, E|
|Other Accession||NP_899646.1, NP_004281.1|
|Calculated MW||53837 Da|
|Antigen Region||336-364 aa|
|Other Names||E3 ubiquitin-protein ligase RNF14, 632-, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein, RNF14, ARA54|
|Target/Specificity||This RNF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human RNF14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Widely expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Xu, K., et al. Cancer Cell 15(4):270-282(2009)
Lan, K.C., et al. Fertil. Steril. 89 (5 SUPPL), 1397-1405 (2008) :
Kikuchi, H., et al. Carcinogenesis 28(8):1752-1758(2007)
Yang, Z., et al. Endocrinology 148(3):1340-1349(2007)
Yang, Z., et al. Mol. Endocrinol. 21(2):343-358(2007)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.