|Application ||WB, E|
|Other Accession||NP_899646.1, NP_004281.1|
|Calculated MW||53837 Da|
|Antigen Region||336-364 aa|
|Other Names||E3 ubiquitin-protein ligase RNF14, 632-, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein, RNF14, ARA54|
|Target/Specificity||This RNF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human RNF14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Xu, K., et al. Cancer Cell 15(4):270-282(2009)
Lan, K.C., et al. Fertil. Steril. 89 (5 SUPPL), 1397-1405 (2008) :
Kikuchi, H., et al. Carcinogenesis 28(8):1752-1758(2007)
Yang, Z., et al. Endocrinology 148(3):1340-1349(2007)
Yang, Z., et al. Mol. Endocrinol. 21(2):343-358(2007)
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