|Application ||WB, E|
|Other Accession||Q9QYB2, NP_542938.2, NP_542937.2|
|Calculated MW||78676 Da|
|Antigen Region||270-297 aa|
|Other Names||Dachshund homolog 1, Dach1, DACH1, DACH|
|Target/Specificity||This DACH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-297 amino acids from the Central region of human DACH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DACH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin- dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain (By similarity).|
|Tissue Location||Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen|
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This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Bailey, S.D., et al. Diabetes Care (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kottgen, A., et al. Nat. Genet. 42(5):376-384(2010)
Zhou, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6864-6869(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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