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ASAH2 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP17664b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP17664b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ASAH2 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q9NR71 |
| Other Accession | P0C7U2, P0C7U1, NP_001072984.1 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85516 Da |
ASAH2 Antibody (C-term) - Additional info | |
| Gene ID 653308 | |
| Other Names ASAH2; HNAC1; Neutral ceramidase; Acylsphingosine deacylase 2; BCDase; LCDase; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form | |
| Target/Specificity This ASAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 733-762 amino acids from the C-terminal region of human ASAH2. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ASAH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ASAH2 Antibody (C-term) - Protein Information | |
| Name ASAH2 | |
| Synonyms HNAC1 | |
| Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract | |
| Cellular Location Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo | |
| Tissue Location Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B | |
ASAH2 Antibody (C-term) - Related products
ASAH2 Antibody (C-term) - Application data
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ASAH2 Antibody (C-term) (Cat. #AP17664b) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).
ASAH2 Antibody (C-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
REFERENCES
Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010) Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007) Ohlsson, L., et al. Biochimie 89(8):950-960(2007) Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007) Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :
