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PET112L Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PET112L Antibody (N-term) AP17705A
    PET112L Antibody (N-term) (Cat. #AP17705a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75879
Other Accession NP_004555.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61864 Da
Antigen Region 34-61 aa
Additional Information
Gene ID 5188
Other Names Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03147}, Glu-AdT subunit B {ECO:0000255|HAMAP-Rule:MF_03147}, 635- {ECO:0000255|HAMAP-Rule:MF_03147}, Cytochrome c oxidase assembly factor PET112 homolog, GATB
Target/Specificity This PET112L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human PET112L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPET112L Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATB
Function Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln).
Cellular Location Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03147, ECO:0000269|PubMed:9878253}
Tissue Location Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart.
Research Areas
Citations (0)

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Background

PET112L furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (Potential).

References

Nagao, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16209-16214(2009)
Petruzzella, V., et al. Genomics 54(3):494-504(1998)

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$ 295.00
$ 99.00
Cat# AP17705A
Size:
Quantity:
(40 western blots)
Availability: In Stock
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