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NFKBIL2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NFKBIL2 Antibody (N-term) AP17737A
    NFKBIL2 Antibody (N-term) (Cat. #AP17737a) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the NFKBIL2 antibody detected the NFKBIL2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96HA7
Other Accession NP_038460.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 150929 Da
Antigen Region 38-65 aa
Additional Information
Gene ID 4796
Other Names Tonsoku-like protein, Inhibitor of kappa B-related protein, I-kappa-B-related protein, IkappaBR, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2, TONSL, IKBR, NFKBIL2
Target/Specificity This NFKBIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human NFKBIL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFKBIL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TONSL {ECO:0000303|PubMed:21055983, ECO:0000312|HGNC:HGNC:7801}
Function Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:21113133, PubMed:26527279, PubMed:27797818, PubMed:29478807, PubMed:27338793, PubMed:30773278). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed:21055983, PubMed:21055984, PubMed:21055985). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA): the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:27797818, PubMed:29478807). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed:27797818, PubMed:29478807). Within the complex, TONSL acts as a histone reader, which recognizes and binds newly synthesized histones following their replacement by histone chaperones (PubMed:29478807, PubMed:27338793). Specifically binds histone H4 lacking methylation at 'Lys-20' (H4K20me0) and histone H3.1 (PubMed:27338793).
Cellular Location Nucleus. Chromosome. Cytoplasm Note=Mainly nuclear (PubMed:21055983, PubMed:21055984). Localizes to DNA damage sites, accumulates at stressed replication forks (PubMed:21055983, PubMed:21055984, PubMed:26527279, PubMed:27338793) Recruited to stalled or collapsed replication forks following histone replacement by histone chaperones ASF1A and the CAF-1 complex: TONSL acts as a histone reader that recognizes and binds newly synthesized histones (PubMed:29478807).
Tissue Location Expressed in heart, skeletal muscle and tracheal epithelial cells.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.

References

Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Norman, D.A., et al. Ann. Hum. Genet. 64 (PT 1), 15-23 (2000) :
Ray, P., et al. J. Biol. Chem. 270(18):10680-10685(1995)

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$ 182.50
$ 70.00
Cat# AP17737A
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