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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ERN1 Antibody (C-term)   

ERN1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ERN1 Antibody (C-term) AP17747b
    ERN1 Antibody (C-term) (Cat. #AP17747b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ERN1 antibody detected the ERN1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75460
Other Accession NP_001424.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 109735 Da
Antigen Region 828-857 aa
Additional Information
Gene ID 2081
Other Names Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, ERN1 (HGNC:3449)
Target/Specificity This ERN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-857 amino acids from the C-terminal region of human ERN1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERN1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERN1 (HGNC:3449)
Function Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Ubiquitously expressed. High levels observed in pancreatic tissue.
Research Areas
Citations (0)

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Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16113-16118(2010)
Auf, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15553-15558(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Gupta, S., et al. PLoS Biol. 8 (7), E1000410 (2010) :

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Cat# AP17747b
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