UAP1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q16222 |
Other Accession | Q91YN5, NP_003106.3 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 58769 Da |
Antigen Region | 390-418 aa |
Gene ID | 6675 |
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Other Names | UDP-N-acetylhexosamine pyrophosphorylase, Antigen X, AGX, Sperm-associated antigen 2, UDP-N-acetylgalactosamine pyrophosphorylase, AGX-1, UDP-N-acetylglucosamine pyrophosphorylase, AGX-2, UAP1, SPAG2 |
Target/Specificity | This UAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of human UAP1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | UAP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | UAP1 {ECO:0000303|PubMed:9603950, ECO:0000312|HGNC:HGNC:12457} |
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Function | Catalyzes the last step in biosynthesis of uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by converting UTP and glucosamine 1-phosphate (GlcNAc-1-P) to the sugar nucleotide (PubMed:9603950, PubMed:9765219). Also converts UTP and galactosamine 1-phosphate (GalNAc-1-P) into uridine diphosphate-N-acetylgalactosamine (UDP-GalNAc) (PubMed:9765219). In addition to its role in metabolism, acts as a regulator of innate immunity in response to virus infection by mediating pyrophosphorylation of IRF3: catalyzes pyrophosphorylation of IRF3 phosphorylated at 'Ser-386' by TBK1, promoting IRF3 dimerization and activation, leading to type I interferon responses (PubMed:36603579). |
Cellular Location | Cytoplasm, cytosol. Note=In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail. |
Tissue Location | Widely expressed (PubMed:8025165). Expressed at low level in placenta, muscle and liver (PubMed:8025165) [Isoform AGX2]: Isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. |
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Provided below are standard protocols that you may find useful for product applications.
Background
UAP1 converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
References
Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Peneff, C., et al. EMBO J. 20(22):6191-6202(2001)
Wang-Gillam, A., et al. J. Biol. Chem. 273(42):27055-27057(1998)
Diekman, A.B., et al. Mol. Reprod. Dev. 50(3):284-293(1998)
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