|Application ||WB, E|
|Calculated MW||48149 Da|
|Antigen Region||8-36 aa|
|Other Names||Selenocysteine lyase, hSCL, SCLY, SCL|
|Target/Specificity||This SCLY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human SCLY.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SCLY Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the decomposition of L-selenocysteine to L- alanine and elemental selenium.|
|Cellular Location||Cytoplasm, cytosol.|
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Provided below are standard protocols that you may find useful for product applications.
Selenocysteine lyase (SCLY; EC 188.8.131.52) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yepes, J.O., et al. Biomedica 26(2):194-205(2006)
Mihara, H., et al. J. Biol. Chem. 275(9):6195-6200(2000)
Daher, R., et al. J Trace Elem Electrolytes Health Dis 6(3):189-194(1992)
Esaki, N., et al. J. Biol. Chem. 257(8):4386-4391(1982)
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