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HIBADH Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HIBADH Antibody (N-term) AP17874a
    HIBADH Antibody (N-term) (Cat. #AP17874a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HIBADH antibody detected the HIBADH protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P31937
Other Accession NP_689953.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35329 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 11112
Other Names 3-hydroxyisobutyrate dehydrogenase, mitochondrial, HIBADH, HIBADH
Target/Specificity This HIBADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIBADH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIBADH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIBADH
Cellular Location Mitochondrion.
Tissue Location Detected in skin fibroblasts.
Research Areas
Citations (0)
citation

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Background

3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Rougraff, P.M., et al. J. Biol. Chem. 264(10):5899-5903(1989)
Rougraff, P.M., et al. J. Biol. Chem. 263(1):327-331(1988)
ROBINSON, W.G., et al. J. Biol. Chem. 225(1):511-521(1957)

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$ 182.50
$ 70.00
Cat# AP17874a
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