HIBADH Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | P31937 |
Other Accession | NP_689953.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 35329 Da |
Antigen Region | 1-30 aa |
Gene ID | 11112 |
---|---|
Other Names | 3-hydroxyisobutyrate dehydrogenase, mitochondrial, HIBADH, HIBADH |
Target/Specificity | This HIBADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIBADH. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HIBADH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HIBADH |
---|---|
Cellular Location | Mitochondrion. |
Tissue Location | Detected in skin fibroblasts. |
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Provided below are standard protocols that you may find useful for product applications.
Background
3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.
References
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Rougraff, P.M., et al. J. Biol. Chem. 264(10):5899-5903(1989)
Rougraff, P.M., et al. J. Biol. Chem. 263(1):327-331(1988)
ROBINSON, W.G., et al. J. Biol. Chem. 225(1):511-521(1957)
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