|Application ||WB, E|
|Calculated MW||35329 Da|
|Antigen Region||1-30 aa|
|Other Names||3-hydroxyisobutyrate dehydrogenase, mitochondrial, HIBADH, HIBADH|
|Target/Specificity||This HIBADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIBADH.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIBADH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Detected in skin fibroblasts.|
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Provided below are standard protocols that you may find useful for product applications.
3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 188.8.131.52) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Rougraff, P.M., et al. J. Biol. Chem. 264(10):5899-5903(1989)
Rougraff, P.M., et al. J. Biol. Chem. 263(1):327-331(1988)
ROBINSON, W.G., et al. J. Biol. Chem. 225(1):511-521(1957)
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