APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||A6NCE7, NP_073729.1|
|Calculated MW||14688 Da|
|Antigen Region||74-106 aa|
|Other Names||Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3|
|Target/Specificity||This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-106 amino acids from the Central region of human APG8b (MAP1LC3B)-T93/Y99.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.|
|Cellular Location||Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme (By similarity)|
|Tissue Location||Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver|
Provided below are standard protocols that you may find useful for product applications.
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
Komatsu, M., et al. Cell 131(6):1149-1163(2007)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Tanida, I., et al. J. Biol. Chem. 279(46):47704-47710(2004)
He, H., et al. J. Biol. Chem. 278(31):29278-29287(2003)
Tanida, I., et al. J. Biol. Chem. 277(16):13739-13744(2002)
Tanida, I., et al. Biochem. Biophys. Res. Commun. 292(1):256-262(2002)
Kabeya, Y., et al. EMBO J. 19(21):5720-5728(2000)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.