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APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - APG8b (MAP1LC3B)-T93/Y99  Antibody (Center) AP1802e
    Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) (Cat. #AP1802e) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.
  • WB - APG8b (MAP1LC3B)-T93/Y99  Antibody (Center) AP1802e
    APG8b (MAP1LC3B)-T93/Y99 (Cat. #AP1802f) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).
  • IHC-P - APG8b (MAP1LC3B)-T93/Y99  Antibody (Center) AP1802e
    APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (Cat. #AP1802e) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - APG8b (MAP1LC3B)-T93/Y99  Antibody (Center) AP1802e
    APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (Cat. #AP1802e) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZQ8
Other Accession A6NCE7, NP_073729.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14688 Da
Antigen Region 74-106 aa
Additional Information
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Target/Specificity This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-106 amino acids from the Central region of human APG8b (MAP1LC3B)-T93/Y99.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPG8b (MAP1LC3B)-T93/Y99 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Research Areas
Citations (0)

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The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.


Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
Komatsu, M., et al. Cell 131(6):1149-1163(2007)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Tanida, I., et al. J. Biol. Chem. 279(46):47704-47710(2004)
He, H., et al. J. Biol. Chem. 278(31):29278-29287(2003)
Tanida, I., et al. J. Biol. Chem. 277(16):13739-13744(2002)
Tanida, I., et al. Biochem. Biophys. Res. Commun. 292(1):256-262(2002)
Kabeya, Y., et al. EMBO J. 19(21):5720-5728(2000)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

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$ 295.00
$ 99.00
Cat# AP1802e
(40 western blots)
Availability: In Stock
Bulk Size
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