|Application ||WB, E|
|Other Accession||Q3UQN2, NP_620137.2|
|Calculated MW||88924 Da|
|Antigen Region||137-165 aa|
|Other Names||FCH domain only protein 2, FCHO2|
|Target/Specificity||This FCHO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the Central region of human FCHO2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FCHO2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP- 2-dependent endocytosis of the transferin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.|
|Cellular Location||Membrane, clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2 (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
FCHO2 may play a role in membrane remodeling by imposing and stabilizing particular membrane curvatures.
Henne, W.M., et al. Structure 15(7):839-852(2007)
Katoh, M., et al. Int. J. Mol. Med. 14(2):327-331(2004)
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