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ATG3 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1807e | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ATG3 Antibody - Product info | |
| Application | IF, WB, IHC
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| Primary Accession | Q9NT62 |
| Other Accession | Q6GQE7, Q6AZ50, Q9CPX6, Q0VCL3 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35864 Da |
ATG3 Antibody - Additional info | |
| Gene ID 64422 | |
| Other Names ATG3; APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; Protein PC3-96 | |
| Target/Specificity This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids surrounding Y18 of human APG3L. | |
| Dilution IF~~1:200 WB~~1:50~100WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG3 Antibody - Protein Information | |
| Name ATG3 | |
| Synonyms APG3, APG3L | |
| Function E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy ATG7 (E1-like enzyme) facilitates this reaction by forming an E1- E2 complex with ATG3 | |
| Cellular Location Cytoplasm. | |
| Tissue Location Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta | |
ATG3 Antibody - Related products
AP1807a: ATG3 Antibody (N-term)
AP1807b: ATG3 Antibody (C-term)
AP1807c: ATG3 Antibody (C-term K183)
AP3430a: Phospho-ATG3-Y18 Antibody
AP3740a: Phospho-APG3 - cleaved Antibody
DC05417: Human ATG3 cDNA Clone
LY11891a: ATG3 Over-expression Lysate
BP1807a: APG3L Antibody (N-term) Blocking Peptide
BP1807b: APG3L Antibody (C-term) Blocking Peptide
BP1807c: APG3L Antibody (C-term K183) Blocking Peptide
BP1807e: APG3L Antibody (Y18) Blocking Peptide
BP1833a: cleaved-APG3 Antibody Blocking Peptide
ATG3 Antibody - Application data
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Fluorescent image of U251 cells stained with ATG3 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1807e ATG3 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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Western blot analysis of Phospho-APG3L-pY18 (Cat. #AP1807e) in T47D cell line lysates (35ug/lane). APG3L (arrow) was detected using the purified Pab.
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Western blot analysis of Phospho-APG3L-pY18 (Cat. #AP1807e) in mouse lung tissue lysates (35ug/lane). APG3L (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APG3L Antibody (Y18) (Cat.#AP1807e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ATG3 Antibody - Research Areas
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BACKGROUND
APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8(MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
REFERENCES
References for protein: 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
