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ATG4B AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1809f | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ATG4B Antibody - Product info | |
| Application | IF, WB, IHC
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| Primary Accession | Q9Y4P1 |
| Other Accession | Q6PZ02 |
| Reactivity | Human |
| Predicted | Chicken |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 44294 Da |
ATG4B Antibody - Additional info | |
| Gene ID 23192 | |
| Other Names ATG4B; APG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B | |
| Target/Specificity This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294~324 amino acids surrounding S309 of human APG4B. | |
| Dilution IF~~1:100 WB~~1:50~100WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG4B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG4B Antibody - Protein Information | |
| Name ATG4B | |
| Synonyms APG4B, AUTL1, KIAA0943 | |
| Function Cysteine protease required for autophagy, which cleaves the C-terminal part of MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Also mediates the lipid deconjugation required for target recycling | |
| Cellular Location Cytoplasm (Probable). | |
| Tissue Location Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas | |
ATG4B Antibody - Related products
AP1809a: ATG4B Antibody (N-term)
AP1809c: ATG4B Antibody (C-term)
AP3560a: Phospho-ATG4B-S121 Antibody
AP3561a: Phospho-ATG4B-S309 Antibody
RI10455: ATG4B predesign siRNA
BP1809a: APG4B Antibody (N-term) Blocking Peptide
BP1809b: APG4B Antibody (G93) Blocking Peptide
BP1809c: APG4B Antibody (C-term) Blocking Peptide
BP1809d: APG4B Antibody (E273) Blocking Peptide
BP1809e: APG4B Antibody (E135) Blocking Peptide
BP1809f: APG4B Antibody (S309) Blocking Peptide
BP1809g: APG4B Antibody (T69) Blocking Peptide
BP1809h: APG4B Antibody (G254) Blocking Peptide
BP1809i: APG4B Antibody (S121) Blocking Peptide
ATG4B Antibody - Application data
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Fluorescent image of U251 cells stained with ATG4B antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1809f ATG4B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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Western blot analysis of anti-APG4B Antibody (S309) (Cat.#AP1809f) in K562 cell line lysates (35ug/lane). APG4B (arrow) was detected using the purified Pab (1:60 dilution).
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Western blot analysis of APG4B (arrow) using rabbit polyclonal APG4B Antibody (S309) (Cat.#AP1809f). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-APG4B-pS309, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ATG4B Antibody - Research Areas
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BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
REFERENCES
References for protein: 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
